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RTLAMP > Advantages
RT-LAMP offers an opportunity to detect SARS-CoV-2 by means that are cheap, simple, and relatively sensitive. Here are some of the advantages of RT-LAMP that make it a good solution for low-resource environments.
For under one Euro for the crude reagent costs of RT-LAMP, the low cost enables massive and rapid testing in almost all parts of the world.
A simple, visual color switch indicates the change in reaction chemistry caused by the presence of the target SARS-CoV-2 molecule in the reaction mix.
Skipping the step of RNA extraction, no need for a thermocycler, no need for fluorescent detection: RT-LAMP reactions run at a constant temperature of 63 degrees and can be carried out in incubators, thermoblocks or even water baths.
Skipping the step of RNA extraction, no need for a thermocycler, no need for fluorescent detection: RT-LAMP reactions run at a constant temperature of 63 degrees and can be carried out in incubators, thermoblocks or even water baths.
We set out to develop a test anyone can use. Our open access protocols utilize open-access enzymes that any molecular biology lab can produce at low cost, circumventing reliance on large companies and bringing down the price per test to incredibly small numbers. Buffer compositions are non-proprietary and public.
Gargling has shown to be an effective, comfortable self sampling method for SARS-CoV-2 testing. The use of this self-sampling method decreases the workload of medical personnel, is more comfortable and has proven to be reliable.
RT-LAMP can be done at home with just a few tubes and a pipette, or in a clinical, high-throughput laboratory with liquid handling robotics, ovens and automatic plate readers. Scalability is a key concern for any real-world impact, and this method can be easily automated and adapted in high-tech clinical praxis.
It doesn’t matter if you have swabs in viral transport medium, universal transport medium or saline, gargle in HBSS, saline or water, RT-LAMP and especially beadLAMP are compatible with a wide variety of sample inputs.
LAMP technology, in and of itself, is very sensitive to contamination. We have implemented a known contamination prevention system into our reactions, based on dUTP and a thermolabile UDG enzyme. This assures that false positives are at a minimum and increases comfort of lab workers.
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